Human IFN alpha ELISA Kit

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

Sweden

ELISA

Interferon alpha

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Human, Non-Human Primates

Colorimetric

2 pg/ml

This ELISA kit detects human IFN alpha subtypes: 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16, and 17.

5-4000 pg/ml

Serum, plasma, and cell culture supernatant.

This Human IFN alpha ELISA Kit applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IFN alpha. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IFN alpha if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound IFN alpha and other components of the sample. A biotinylated Anti-IFN alpha detection antibody is then added followed by Streptavidin-HRP. Addition of TMB substrate will result in a colored substrate product. The reaction is stopped with sulfuric acid and the optical density can be quantified using an ELISA plate reader. The concentration of analyte is determined by comparison to a serial dilution of the ELISA standard in parallel.

Sandwich (quantitative)

Microplate