Merlin (phospho Ser518) Cell Based ELISA Kit

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

China

c-Met

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Human, Mouse, Rat

4 hours 30 minutes

Colorimetric

> 5000 Cells

Adherent cells and suspension cells.

This Merlin (phospho Ser518) Cell Based ELISA Kit allows for the detection of Merlin (phospho Ser518) and the effects that certain stimulation conditions have on Merlin (phospho Ser518) expression in different cell lines. Qualitative determination of Merlin (phospho Ser518) concentration is achieved by an indirect ELISA format. In essence, the Merlin (phospho Ser518) is captured by Anti-Merlin (phospho Ser518) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Merlin (phospho Ser518) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-Merlin Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Cell-based (qualitative)

Microplate