16S rRNA sequencing is a routine technique for microbiome composition profiling. Compared to shotgun metagenomics sequencing, 16S rRNA sequencing is more cost-effective and more robust; it generally requires less input DNA and is less impacted by the presence of host DNA. However, 16S rRNA sequencing has its own challenges. One major challenge is the formation of PCR chimeric sequences, which are artificial sequences resulting from the recombination of two or more PCR templates. Additionally, with common 16S primers, it is difficult to achieve both species-level resolution and broad phylogenetic...
16S rRNA sequencing is a routine technique for microbiome composition profiling. Compared to shotgun metagenomics sequencing, 16S rRNA sequencing is more cost-effective and more robust; it generally requires less input DNA and is less impacted by the presence of host DNA. However, 16S rRNA sequencing has its own challenges. One major challenge is the formation of PCR chimeric sequences, which are artificial sequences resulting from the recombination of two or more PCR templates. Additionally, with common 16S primers, it is difficult to achieve both species-level resolution and broad phylogenetic...
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Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
All orders received before 3:00 pm Pacific Standard Time Monday through Friday will be shipped the same day via FedEx® Ground delivery unless otherwise stated or requested.
COUNTRY OF ORIGIN
United States of America
*USAGE / SAFETY STATEMENT
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
INPUT
Purified microbial DNA ≤ 20 ng/µl, free of PCR inhibitors.
Never met such a great alternative to cost-effective NGS library preparation reagents like this from Zymo research. Awesome and simplifyed workflow which makes...
Jose
Argenomics and Fundacion Investigar
This kit is extremely easy to use and the technical support from Zymo was extremely helpful. I highly recommend.
Matthew
San Diego State University
The Quick 16s amplicon library prep kit is extremely time saving compared to the in house library prep protocol that we have used before. Quality of the sequencing...
Duleepa
Korea University
Never met such a great alternative to cost-effective NGS library preparation reagents like this from Zymo research. Awesome and simplifyed workflow which makes...
Jose
Argenomics and Fundacion Investigar
This kit is extremely easy to use and the technical support from Zymo was extremely helpful. I highly recommend.
Matthew
San Diego State University
The Quick 16s amplicon library prep kit is extremely time saving compared to the in house library prep protocol that we have used before. Quality of the sequencing...
Duleepa
Korea University
Pleased to inform the community of this great 16S sequencing library prep kit.
Jose R.
Argenomics and Fundacion Investigar
Never met such a great alternative to cost-effective NGS library preparation reagents like this from Zymo research. Awesome and simplifyed workflow which makes things way easier.
Easy to use and great technical support.
Matthew V.
San Diego State University
This kit is extremely easy to use and the technical support from Zymo was extremely helpful. I highly recommend.
Saves a lot of time and results are highly reproducible.
Duleepa P.
Korea University
The Quick 16s amplicon library prep kit is extremely time saving compared to the in house library prep protocol that we have used before. Quality of the sequencing read was increased significantly with the kit. Kit comes with the qPCR master mix that enables us to limit the PCR amplification to minimum number of PCR cycles and reduce the PCR bias. And the qPCR also can be used to quantity the samples at the same time with library amplification. This saves a lot of time necessary to quantify individual samples. This makes the kit ideal for high throughput experiments. The enzymatic cleanup eliminates the necessity of PCR cleanup after 16s target amplification.
Overall this kit is time saving, highly efficient and generates reproducible data. We tested this with Zymo Research community DNA standards and the results were highly reproducible. This kit is the best kit available in the market for 16s amplicon sequencing.
Citations (0)
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
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