The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
All orders received before 3:00 pm Pacific Standard Time Monday through Friday will be shipped the same day via FedEx® Ground delivery unless otherwise stated or requested.
COUNTRY OF ORIGIN
United States of America
*USAGE / SAFETY STATEMENT
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
SOURCE
Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
I used this kit to generate a bisulfite-converted genome. The protocol is very simple, and faster and the amount of recovered converted DNA is almost 80% of the...
Sivaraman
St jude childrens research hospital
The kits is easy to use even for the novice in methylation research field.
The consistency between the repeat samples was very great and the accuracy is also very...
BO
PROMIS DIAGNOSTICS
This product was used for a triple negative breast cancer cell line study evaluating the efficacy of various drug treatments including JQ1 and TSA.
The best...
Andrew
Center for Functional Genomics
This is great kit. Since bisulfite conversion is a very delicate process, this kit provided me with high quality bisulfite DNA, suitable for methylation analysis...
Ritchie
LUMC
This kit made the preparation for pyrosequencing easy and straightforward. The instructions are simple and easy to learn when I first started using this kit.
Juliann
University of California, Davis
The EZ DNA Methylation-Gold Kit is really easy to use and very efficient. Robust and reproductive conversion. The after-sales care is good too, not really necessary...
Vincent
Columbia Universtity
I used this kit to generate a bisulfite-converted genome. The protocol is very simple, and faster and the amount of recovered converted DNA is almost 80% of the...
Sivaraman
St jude childrens research hospital
The kits is easy to use even for the novice in methylation research field.
The consistency between the repeat samples was very great and the accuracy is also very...
BO
PROMIS DIAGNOSTICS
This product was used for a triple negative breast cancer cell line study evaluating the efficacy of various drug treatments including JQ1 and TSA.
The best...
Andrew
Center for Functional Genomics
This is great kit. Since bisulfite conversion is a very delicate process, this kit provided me with high quality bisulfite DNA, suitable for methylation analysis...
Ritchie
LUMC
This kit made the preparation for pyrosequencing easy and straightforward. The instructions are simple and easy to learn when I first started using this kit.
Juliann
University of California, Davis
The EZ DNA Methylation-Gold Kit is really easy to use and very efficient. Robust and reproductive conversion. The after-sales care is good too, not really necessary...
Vincent
Columbia Universtity
Fast and easy to use
Sivaraman N.
St jude childrens research hospital
I used this kit to generate a bisulfite-converted genome. The protocol is very simple, and faster and the amount of recovered converted DNA is almost 80% of the input DNA.
Methylation study is easy!
BO B.
PROMIS DIAGNOSTICS
The kits is easy to use even for the novice in methylation research field.
The consistency between the repeat samples was very great and the accuracy is also very high.
When I have question related to the kit, the technical support team and sales rep quickly respond and solve the questions.
Best methyl-seq data ever!
Andrew H.
Center for Functional Genomics
This product was used for a triple negative breast cancer cell line study evaluating the efficacy of various drug treatments including JQ1 and TSA.
The best aspect of this kit is it worked the first time right out of the box. Following the manufacturers protocol directly the kit yielded approximately 150 ng of bisulfite treated DNA for direct input to Accel-NGS methylseq DNA library preparation. All libraries looked uniform by Bioanalyzer and Qubit/qPCR quantification. Data from this study showed clear distinctions between treatments and untreated samples.
The product was extremely easy to use. The supplied protocol was thorough, precise and correct. The results were highly reproducible and exceptional. The value for money couldnt be beat!
Citations (0)
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.