Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c- terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enables a selective identification and purification of the protein of interest. Poly-His (His x 6) containing proteins can be purified by immobilized metal ion affinity chromatography (IMAC), making use of its high affinity for transition metal ions (1-4). This purification system eliminates the harsh conditions required to elute protein from ligand affinity...
Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c- terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enables a selective identification and purification of the protein of interest. Poly-His (His x 6) containing proteins can be purified by immobilized metal ion affinity chromatography (IMAC), making use of its high affinity for transition metal ions (1-4). This purification system eliminates the harsh conditions required to elute protein from ligand affinity...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Epitope Tag, His
APPLICATION
IP
CLONE NUMBER
11C113
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified
PHYSICAL FORM
Supplied as a liquid in PBS, 0.05% azide as 1:1 (v/v) suspension. Labeled with Sepharose. Binding capacity is at least 5-10nmoles of HIS tagged protein per 1ml settled resin.
GRADE
Purified
ANTIBODY TYPE
Mab
ISOTYPE
IgG
IMMUNOGEN
His-tag-fusion protein.
SPECIFIC ACTIVITY
Recognizes native and denatured-reduced forms of synthetic poly-histidine or poly-histidine-tagged fusion proteins.
COMMODITY CODE
30021010
HOST
mouse
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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