Tribioscience’s NNMT inhibitor screening kit is a fluorometric assay that utilizes SAM and nicotinamide as the substrates to generate SAH which is hydrolyzed to form homocysteine. The free thiol group of the homocysteine can be detected with a thiol probe generating an enhanced fluorescent signal at Ex/Em = 400/465 nm. In the presence of an NNMT inhibitor, the fluorescent signal is reduced in concentration dependent manner. The kit provides the easiest, most accurate, and simplest approach for high-throughput screening of NNMT inhibitors.
Tribioscience’s NNMT inhibitor screening kit is a fluorometric assay that utilizes SAM and nicotinamide as the substrates to generate SAH which is hydrolyzed to form homocysteine. The free thiol group of the homocysteine can be detected with a thiol probe generating an enhanced fluorescent signal at Ex/Em = 400/465 nm. In the presence of an NNMT inhibitor, the fluorescent signal is reduced in concentration dependent manner. The kit provides the easiest, most accurate, and simplest approach for high-throughput screening of NNMT inhibitors.
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