DBD-COCl [=4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]

HPLC Derivatization Reagents

Japan

For laboratory research and development purposes only.

>93.0%(T)

HPLC Labeling Reagent The compound 1 is an HPLC fluorescence labeling reagent, which reacts with many kinds of nucleophilic groups under mild conditions. The reaction examples are shown in the table below. These resulting compounds are stable, and can reach the detector without any decomposition under reversed phase HPLC, thus excellent chromatograms can be obtained by fluorescence detection. Groups Examples Reaction Conditions Wavelengths (nm) Detection Limits (fmol) ex em Alcohols Androsterone 60℃, 30 min 443 546 38 α-Oxyacids Mandelic acid 60℃, 15 min 442 551 125 Phenols Estrone 60℃, 15 min 440 543 40 Amines Benzylamine r.t. or 60℃, 15 min 445 555 89 Aromatic amines Phenetidine 60℃, 15 min 443 553 56 Thiols 2-Mercapto-N-(2-naphthyl)acetamide r.t. 437 544 103 Application examples: 10 µL of 25 mM labeling reagent 1 in dry benzene is mixed with 10 µL of 0.5 mM androsterone in dry benzene (containing 0.5 mM quinuclidine＊), and incubated at 60 °C for 30 min. The reaction solution is quenched with 980 µL of 50% acetonitrile solution containing 1% acetic acid, use it as the HPLC sample solution. ＊For primary alcohols, quinuclidine is not necessarily needed. TCI Products Pamphlet HPLC Labeling Reagents ( PDF 10MB )

C11H13ClN4O4S

Solid

332.76

Fukaya factory is certified for GMP, ISO14001:2004 (JQA-EM5386) and IQ Net (JP-JQA-EM5386).

P501 : Dispose of contents/ container to an approved waste disposal plant. P260 : Do not breathe dusts or mists. P234 : Keep only in original container. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection. P390 : Absorb spillage to prevent material damage. P303 + P361 + P353 : IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower. P301 + P330 + P331 : IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. P363 : Wash contaminated clothing before reuse. P304 + P340 + P310 : IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor. P305 + P351 + P338 + P310 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor. P406 : Store in corrosive resistant container with a resistant inner liner. P405 : Store locked up.

Fluorescence Detection Labeling Reagents (for Hydroxy Groups) [HPLC Derivatization Reagents]

MFCD00191377

GHS05

8

UN3261

Light yellow to Yellow to Orange powder to crystal

87563176.0

Danger

H314 : Causes severe skin burns and eye damage. H290 : May be corrosive to metals.

III

2934.10.9000

>93.0%(T)

min. 93.0 %