Specifications
SHIPPING NOTES
Blue Ice or 4ºC. All orders received in North America by 12:00 PM PST Monday to Thursday will usually be shipped out on the same day. North American customers will typically receive their orders within 1-2 business days. All shipments are F.O.B. from point of shipment, pre-paid and added to the invoice. Freight charges include but not limited to: priority service and packaging materials.Products are shipped with “cold packs” in insulated containers. There is a charge for this packaging and for the shipping costs via FedEx. Please inspect packages immediately upon receipt and inform Labscoop Customer Support of any shortage or damage within 48 hours of receipt. We can only accept returns after we have given prior authorization and issued specific shipping instructions. If a customer wishes to use their own FedEx account number please indicate this on the purchase order or during checkout.
*USAGE / SAFETY STATEMENT
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
ANTIBODY TYPE
WB (1:5000); optimal dilutions for assays should be determined by the user.
REACTIVITY SPECIES
Human | Mouse | Rat
QUALITY CERTIFICATION / COMPLIANCE
ISO 9001:2015
IMMUNOGEN
Prepared by immunizing synthetic peptide coupled to KLH.
SPECIFICITY
Detects endogenous Histone H3(tri methyl K36) protein.
STORAGE BUFFER
PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide *Storage buffer may change when conjugated
ANTIBODY ID
https://antibodyregistry.org/search.php?q=AB_2699666
CELLUAR LOCALIZATIONS
Nucleus | Chromosome
SCIENTIFIC BACKGROUND
Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1. Histones play a cental role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
REFERENCES
1. Wu R., et al. (1981) Cell. 27:321.
2. Strahl B., et al. (2000) Nature. 403:41.
3. Bauer U., et al. (2002) EMBO Rep. 3:39.
4. Frank D., et al. (2003) Gene. 312:135.
RESEARCH AREA
Cell Signaling | Epigenetics and Nuclear Signaling | Histones | H3 | H3 Acetylation
ALTERNATIVE NAMES
H3 histone family, member A Antibody, H3/A Antibody, H31_HUMAN Antibody, H3FA Antibody, Hist1h3a Antibody, histone 1 H3a Antibody, Histone cluster 1 H3a Antibody, Histone H3.1 Antibody
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