CPK21 / Anti-Calcium-Dependent Protein Kinase 21 Antibody

Primary Antibodies

Arabidopsis Antibodies

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

United States

Research Use Only.

Lyophilized

60 kDa

Rabbit Polyclonal Antibody

Serum

Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.

KLH-conjugated synthetic peptide (14 aa from Central section) derived from Arabidopsis thaliana CPK21 (AT4G04720).(AT4G04720, Q9ZSA2)

Arabidopsis thaliana, Brassica rapa, Brassica napus, Gossypium raimondii, Nicotiana tabacum, Populus trichocarpa, Zea mays, Sorghum bicolor, Setaria viridis, Medicago truncatula, Solanum tuberosum, Glycine max, Solanum lycopersicum, Cucumis sativus, Oryza sativa Indica Group, Oryza sativa Japonica Group, Triticum aestivum, Hordeum vulgare

Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

60 kDa

Recom: 2.5 ng, 10 ng and 25 ng recombinant protein containing the peptide for immunization and having a molecular mass of 55 kDa. Electrophoresis: 12% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000). Detection: using chemiluminescence substrate and image were captured with CCD camera.