PsbO is a subunit of the oxygen evolving complex of Photosystem II, encoded by the PsbO (sll0427). In Arabidopsis thaliana, the PsbO proteins are encoded by two genes: PsbO1 (AT5G66570) and PsbO2 (AT3G50820). PsbO1 is the major isoform in Arabidopsis.
PsbO is a subunit of the oxygen evolving complex of Photosystem II, encoded by the PsbO (sll0427). In Arabidopsis thaliana, the PsbO proteins are encoded by two genes: PsbO1 (AT5G66570) and PsbO2 (AT3G50820). PsbO1 is the major isoform in Arabidopsis.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Safety Statement
Research Use Only.
Regulatory & Compliance
Specifications
CLASS
Primary Antibodies
SUBCLASS
Algae Antibodies
SHIPPING NOTES
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
COUNTRY OF ORIGIN
United States
*USAGE / SAFETY STATEMENT
Research Use Only.
PHYSICAL FORM
Lyophilized
FREE CHARACTERISTICS
30.4 / 35 kDa
CLONALITY
Rabbit Polyclonal Antibody
PURIFICATION
Serum
DILUTION
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
IMMUNOGEN
Recombinant protein of PsbO (1-274aa) derived from Synechocystis sp. PCC 6803 (sll0427).(sll0427, P10549)
RECONSTITUTION
Reconstitution with 150 µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
EXPECTED / APPARENT MW
30.4 / 35 kDa
APPLICATION EXAMPLE
PCC 6803-Thy is thylakoid membrane protein from Synechocystis sp. PCC 6803 cells containing 2.5 μg and 5 μg of protein, respectively. Electrophoresis: 12% SDS-Urea-PAGE. Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT for 1h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (Cat# PHY6000). Detection: using chemiluminescence substrate and image were captured with CCD camera.
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Safety Statement
Research Use Only.
Regulatory & Compliance
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