NMT1 / Anti-Glycylpeptide N-Tetradecanoyltransferase 1 Antibody

Primary Antibodies

Arabidopsis Antibodies

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

United States

Research Use Only.

Lyophilized

50 kDa

Rabbit Polyclonal Antibody

Serum

Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.

KLH-conjugated synthetic peptide (16 aa from N terminal section) derived from Arabidopsis thaliana NMT1 (AT5G57020).(AT5G57020, Q9LTR9)

Glycine, max, Populus trichocarpa, Brassica napus, Brassica rapa, Gossypium raimondii, Medicago truncatula, Vitis vinifera, Spinacia oleracea, Solanum, tuberosum, Nicotiana tabacum, Cucumis sativus, Panicum virgatum, Hordeum vulgare, Triticum aestivum, Setaria viridis, Physcomitrium, patens, Chlamydomonas reinhardtii, Solanum lycopersicum, Oryza, sativa, Sorghum bicolor, Zea mays

Arabidopsis thaliana

Reconstitution with 150 µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

50 kDa

Example1: Mito: 10 μg mitochondria protein from Arabidopsis thaliana. Electrophoresis: 15% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000). Detection: using chemiluminescence substrate and image were captured with CCD camera. Example2: Recom: 2.5 ng, 10 ng and 25 ng recombinant protein containing the peptide for immunization and having a molecular mass of 43 kDa. Electrophoresis: 12% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000). Detection: using chemiluminescence substrate and image were captured with CCD camera.