NMT1 / Anti-Glycylpeptide N-Tetradecanoyltransferase 1 Antibody
PhytoAB Inc.
SKU / CAT#: PHY1851S
$28900
usd$1.93/μg
NMT1 / Anti-Glycylpeptide N-Tetradecanoyltransferase 1 Antibody
PHYTOAB INC.SKU / CAT#: PHY1851S
MPN: PHY1851S
$28900
usd$1.93/μg
Available
SHIPS May 26th
Available
SHIPS May 26th
SKU
Unit size / Options
Availability
Price
No products found
Description
Arabidopsis thaliana, like human, has two tightly regulated N-myristoyltransferase (NMT) genes, NMT1 (AT5G57020) and NMT2 (AT2G44170). The AtNMT1 expression profile indicated ubiquity in roots, stem, leaves, flowers, and siliques (approximately 1.7 kb transcript and approximately 50 kDa immunoreactive polypeptide) but a greater level in the younger tissue, which are developmentally very active.
Arabidopsis thaliana, like human, has two tightly regulated N-myristoyltransferase (NMT) genes, NMT1 (AT5G57020) and NMT2 (AT2G44170). The AtNMT1 expression profile indicated ubiquity in roots, stem, leaves, flowers, and siliques (approximately 1.7 kb transcript and approximately 50 kDa immunoreactive polypeptide) but a greater level in the younger tissue, which are developmentally very active.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Safety Statement
Research Use Only.
Regulatory & Compliance
Technical Documents
Specifications
CLASS
Primary Antibodies
SUBCLASS
Arabidopsis Antibodies
SHIPPING NOTES
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
COUNTRY OF ORIGIN
United States
*USAGE / SAFETY STATEMENT
Research Use Only.
PHYSICAL FORM
Lyophilized
FREE CHARACTERISTICS
50 kDa
CLONALITY
Rabbit Polyclonal Antibody
PURIFICATION
Serum
DILUTION
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
IMMUNOGEN
KLH-conjugated synthetic peptide (16 aa from N terminal section) derived from Arabidopsis thaliana NMT1 (AT5G57020).(AT5G57020, Q9LTR9)
PREDICTED SPECIFICITY & CROSS REACTIVITY
Glycine, max, Populus trichocarpa, Brassica napus, Brassica rapa, Gossypium raimondii, Medicago truncatula, Vitis vinifera, Spinacia oleracea, Solanum, tuberosum, Nicotiana tabacum, Cucumis sativus, Panicum virgatum, Hordeum vulgare, Triticum aestivum, Setaria viridis, Physcomitrium, patens, Chlamydomonas reinhardtii, Solanum lycopersicum, Oryza, sativa, Sorghum bicolor, Zea mays
CONFIRMED SPECIFICITY & CROSS REACTIVITY
Arabidopsis thaliana
RECONSTITUTION
Reconstitution with 150 µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
EXPECTED / APPARENT MW
50 kDa
APPLICATION EXAMPLE
Example1: Mito: 10 μg mitochondria protein from Arabidopsis thaliana. Electrophoresis: 15% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000). Detection: using chemiluminescence substrate and image were captured with CCD camera. Example2: Recom: 2.5 ng, 10 ng and 25 ng recombinant protein containing the peptide for immunization and having a molecular mass of 43 kDa. Electrophoresis: 12% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000). Detection: using chemiluminescence substrate and image were captured with CCD camera.
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Safety Statement
Research Use Only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.Join Our List
Subscribe to the Scoop blog and get the latest updates on life in the lab.
Related Blog Articles
Related Blog Articles