The hygromycin phosphotransferase (denoted hpt, hph or aphIV) gene was originally derived from Escherichia coli. The gene codes for hygromycin phosphotransferase (HPT), which detoxifies the aminocyclitol antibiotic hygromycin B. A large number of plants have been transformed with the hpt gene and hygromycin B has proved very effective in the selection of a wide range of plants, including monocotyledonous. Most plants exhibit higher sensitivity to hygromycin B than to kanamycin, for instance cereals.
The hygromycin phosphotransferase (denoted hpt, hph or aphIV) gene was originally derived from Escherichia coli. The gene codes for hygromycin phosphotransferase (HPT), which detoxifies the aminocyclitol antibiotic hygromycin B. A large number of plants have been transformed with the hpt gene and hygromycin B has proved very effective in the selection of a wide range of plants, including monocotyledonous. Most plants exhibit higher sensitivity to hygromycin B than to kanamycin, for instance cereals.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
COUNTRY OF ORIGIN
United States
*USAGE / SAFETY STATEMENT
Research Use Only.
PHYSICAL FORM
Lyophilized
FREE CHARACTERISTICS
38 kDa
CLONALITY
Mouse Monoclonal Antibody
PURIFICATION
Protein A Purified
DILUTION
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
IMMUNOGEN
Synthetic peptide (18 aa from C terminal section) derived from HPT (ABI30072.1).(ABI30072.1, P00557)
RECONSTITUTION
Reconstitution with 150 µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
EXPECTED / APPARENT MW
38 kDa
APPLICATION EXAMPLE
Recom: 2.5 ng, 10 ng and 25 ng recombinant protein containing the peptide for immunization and having a molecular mass of 40 kDa. Electrophoresis: 12% SDS-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1 h. Blocking: 5% skim milk at RT or 4℃ for 1 h. Primary antibody: 1:1000 dilution overnight at 4℃. Secondary antibody: 1:5000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6006). Detection: using chemiluminescence substrate and image were captured with CCD camera.
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Safety Statement
Research Use Only.
Regulatory & Compliance
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