MURASHIGE & SKOOG MEDIUM  (W/ VITAMINS & CALCIUM CHLORIDE, W/O SUCROSE, AGAR), 5L

Plant Tissue Culture

Plant Culture Media

United States

Dissolve 4.54gms of dehydrated medium in 600ml of distilled or deionized water at room temperature (15-30*C). Rinse media vial with small quantity of distilled water to remove traces of power. Add the desired heat stable supplements prior to autoclaving. Continue stirring until the powder has dissolved. Some times media does not dissolve completely unless the pH is reduced. For these, lower the pH to about 3.0 to facilitate dissolution of media. The pH of medium is adjusted by using 1N HCL/ 1N NaOH/ 1NKOH. Make up the final volume to 1000ml with distilled water. Mix Gently, heat and rotate between intervals until the solution becomes clear. Do not boil, reheat and allow to cool below 50*C during dispensing. Dispense the medium into suitable containers, plug or cap, then autoclave at 15pso (121*C) for 15 minutes, using a slow exhaust cycle. Higher temperatures and/or longer times are not recommended. Cool the autoclaved culture vessels containing medium to 45-50*C and aseptically add desired sterile heat-labile substrate.

This product is intended primarily for laboratory Research Use Only (RUO) and, unless otherwise stated on product labels, are not to be used for any other purposes, including but not limited to, in vitro diagnostic purposes, in foods, drugs, medical devices or cosmetics for humans or animals or for commercial purposes.

White to light tan with homogenous mixture of free flowing powder

Colorless to slight yellow solution, clear, complete