RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic...
RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic...
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Shipped In
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Safety & Storage
Storage Temperature
Store the phospho-RNA polymerase II antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This phospho-RNA polymerase II antibody is available for research use only.
This phospho-RNA polymerase II antibody is available for research use only.
FORMAT
Purified (Sodium Azide Free)
APPLICATION.1
FACS, IF, WB, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgM, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
PEG precipitation
IMMUNOGEN
The immunogen for this phospho-RNA polymerase II antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-S5.
STORAGE BUFFER
1 mg/ml in 1X PBS; BSA free, sodium azide free
APPLICATION DETAILS
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the recombinant phospho-RNA polymerase II antibody should be determined by the researcher.<br><br>1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Nuclear
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the phospho-RNA polymerase II antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This phospho-RNA polymerase II antibody is available for research use only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.