Heat shock protein 90kDa beta member 1, known also as endoplasmin, gp96, grp94 and ERp99, is an HSP90 paralogue that is found in the endoplasmic reticulum. It plays critical roles in folding proteins in the secretory pathway such as Toll-like receptors and integrins. It has been implicated as an essential immune chaperone to regulate both innate and adaptive immunity. [Wiki]

Shipping & Handling

Shipped In

Cold Packs

Safety & Storage

Storage Temperature

Store the GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Safety Statement

This GRP94 antibody is available for research use only.

Regulatory & Compliance

Technical Documents

Datasheet (1)

aWTxEgrp94-antibody-srpr90b-v7173

Specifications

CLASS

Primary Antibodies

SUBCLASS

Monoclonal

UNIPROT ID

P14625

CLONE NUMBER

SRPR90b

*USAGE / SAFETY STATEMENT

This GRP94 antibody is available for research use only.

FORMAT

Purified

APPLICATION.1

IHC-P

HOST SPECIES

Rat

ISOTYPE

Rat IgG2a, kappa

REACTIVITY SPECIES

Human

CLONALITY

Monoclonal (rat origin)

PURIFICATION

Protein G affinity chromatography

IMMUNOGEN

Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.

STORAGE BUFFER

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

APPLICATION DETAILS

Immunohistology (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

APPLICATION NOTES

Titering of the GRP94 antibody may be required for optimal performance.<br><br>1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

LOCALIZATION

Cytoplasmic and nuclear