It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate....
It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate....
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the recombinant TRAcP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This recombinant TRAcP antibody is available for research use only.
This recombinant TRAcP antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
IHC-P
HOST SPECIES
Rabbit
ISOTYPE
Rabbit IgG, kappa
REACTIVITY SPECIES
Human, Mouse, Rat
CLONALITY
Recombinant Rabbit Monoclonal
PURIFICATION
Protein A affinity chromatography
IMMUNOGEN
Recombinant full-length human protein was used as the immunogen for the recombinant TRAcP antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the recombinant TRAcP antibody should be determined by the researcher.<p>1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Cytoplasmic (lysosomes)
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the recombinant TRAcP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This recombinant TRAcP antibody is available for research use only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.