Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order...
Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order...
Shipping & Handling
Shipped In
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Safety & Storage
Storage Temperature
Store the Histone H1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Histone H1 antibody is available for research use only.
This Histone H1 antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
IF, IHC-P, WB, FACS
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2a, kappa
REACTIVITY SPECIES
Human, Rat
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Recombinant full-length human protein was used as the immunogen for the Histone H1 antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
APPLICATION NOTES
Optimal dilution of the Histone H1 antibody should be determined by the researcher.<p>1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Nuclear
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Histone H1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This Histone H1 antibody is available for research use only.
Regulatory & Compliance
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We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.