This mAb preferably reacts with determinants of chain A and H type 3 (Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This mAb is applicable for tissue staining in tumor patients with blood groups A and AB. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells....
This mAb preferably reacts with determinants of chain A and H type 3 (Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This mAb is applicable for tissue staining in tumor patients with blood groups A and AB. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells....
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the ABO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This ABO antibody is available for research use only.
This ABO antibody is available for research use only.
FORMAT
Purified (Sodium Azide Free)
APPLICATION.1
Agglutination, IF, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgM, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
PEG precipitation
IMMUNOGEN
A mixture of erythrocytes of blood group A and glycoprotein fraction isolated from the saliva of secretors with blood group A was used as the immunogen for the ABO antibody.
STORAGE BUFFER
1 mg/ml in 1X PBS; BSA free, sodium azide free
APPLICATION DETAILS
Agglutination (order BSA/sodium azide-free format),Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
APPLICATION NOTES
Optimal dilution of the ABO antibody should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
LOCALIZATION
Cell surface
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the ABO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Safety Statement
This ABO antibody is available for research use only.
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.