This antibody recognizes a protein of 33-37kDa, identified as CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists...
This antibody recognizes a protein of 33-37kDa, identified as CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists...
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Safety & Storage
Storage Temperature
Anti-CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Safety Statement
This anti-CD20 antibody is available for research use only.
This anti-CD20 antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
FACS, IF, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2a, kappa
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
Recombinant human MS4A1/CD20 protein was used as the immunogen for this anti-CD20 antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
APPLICATION NOTES
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-CD20 antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Predominantly cell surface with some cytoplasmic
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Anti-CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Safety Statement
This anti-CD20 antibody is available for research use only.
Regulatory & Compliance
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