Specifications
GENE ID
https://www.ncbi.nlm.nih.gov/gene/Unknown
*USAGE / SAFETY STATEMENT
This Mitochondria marker antibody is available for research use only.
APPLICATION.1
FACS, IF, WB, IHC-P
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity chromatography
IMMUNOGEN
The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondria marker antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
APPLICATION DETAILS
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
APPLICATION NOTES
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Mitochondria marker antibody to be titered up or down for optimal performance.<p>1. Staining of FFPE tissues is enhanced by boiling sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
LOCALIZATION
Mitochondria in cytoplasm
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