USP25 Antibody
SKU / CAT#: RQ6017
$41900
usd
$4.19/UG
RQ6017

USP25 Antibody

NSJ Bioreagents
$41900
usd
$4.19/UG
Available
SHIPS Apr 28th
Available
SHIPS Apr 28th
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Description
Ubiquitin specific peptidase 25 is a protein that in humans is encoded by the USP25 gene. It is mapped to 21q21.1. Ubiquitin is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25.
Ubiquitin specific peptidase 25 is a protein that in humans is encoded by the USP25 gene. It is mapped to 21q21.1. Ubiquitin is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25.
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Safety & Storage
Storage Temperature
After reconstitution, the USP25 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Safety Statement
This USP25 antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Specifications
CLASS
Primary Antibodies
SUBCLASS
Polyclonal
UNIPROT ID
Q9UHP3
*USAGE / SAFETY STATEMENT
This USP25 antibody is available for research use only.
FORMAT
Antigen affinity purified
APPLICATION.1
WB, IF, FACS, Direct ELISA
HOST SPECIES
Rabbit
ISOTYPE
Rabbit IgG
REACTIVITY SPECIES
Human, Mouse, Rat, Monkey
CLONALITY
Polyclonal (rabbit origin)
PURIFICATION
Affinity purified
IMMUNOGEN
Recombinant human protein (amino acids M1-D1053) was used as the immunogen for the USP25 antibody.
STORAGE BUFFER
0.5mg/ml if reconstituted with 0.2ml sterile DI water
APPLICATION DETAILS
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
APPLICATION NOTES
Optimal dilution of the USP25 antibody should be determined by the researcher.
LOCALIZATION
Nuclear, cytoplasmic
BUFFER
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
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Immunofluorescent staining of FFPE human A549 cells with USP25 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human A549 cells with USP25 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HEK293, 2) human placenta, 3) monkey liver, 4) monkey kidney, 5) human PC-3, 6) human HeLa, 7) human A549, 8) rat kidney, 9) rat liver, 10) rat lung, 11) rat PC-12, 12) mouse lung, 13) mouse RAW264.7 and 14) mouse NIH 3T3 lysate with USP25 antibody. Predicted molecular weight ~122 kDa.
Flow cytometry testing of human HL60 cells with USP25 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= USP25 antibody.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
After reconstitution, the USP25 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Safety Statement
This USP25 antibody is available for research use only.
Regulatory & Compliance
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