Ubiquitin specific peptidase 25 is a protein that in humans is encoded by the USP25 gene. It is mapped to 21q21.1. Ubiquitin is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25.

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Safety & Storage

Storage Temperature

After reconstitution, the USP25 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Safety Statement

This USP25 antibody is available for research use only.

Regulatory & Compliance

Technical Documents

Datasheet (1)

xMYVousp25-antibody-rq6017

Specifications

CLASS

Primary Antibodies

SUBCLASS

Polyclonal

UNIPROT ID

Q9UHP3

*USAGE / SAFETY STATEMENT

This USP25 antibody is available for research use only.

FORMAT

Antigen affinity purified

APPLICATION.1

WB, IF, FACS, Direct ELISA

HOST SPECIES

Rabbit

ISOTYPE

Rabbit IgG

REACTIVITY SPECIES

Human, Mouse, Rat, Monkey

CLONALITY

Polyclonal (rabbit origin)

PURIFICATION

Affinity purified

IMMUNOGEN

Recombinant human protein (amino acids M1-D1053) was used as the immunogen for the USP25 antibody.

STORAGE BUFFER

0.5mg/ml if reconstituted with 0.2ml sterile DI water

APPLICATION DETAILS

Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml

APPLICATION NOTES

Optimal dilution of the USP25 antibody should be determined by the researcher.

LOCALIZATION

Nuclear, cytoplasmic

BUFFER

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

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Citations (0)

Immunofluorescent staining of FFPE human A549 cells with USP25 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human HEK293, 2) human placenta, 3) monkey liver, 4) monkey kidney, 5) human PC-3, 6) human HeLa, 7) human A549, 8) rat kidney, 9) rat liver, 10) rat lung, 11) rat PC-12, 12) mouse lung, 13) mouse RAW264.7 and 14) mouse NIH 3T3 lysate with USP25 antibody. Predicted molecular weight ~122 kDa.

Flow cytometry testing of human HL60 cells with USP25 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= USP25 antibody.

Shipping & Handling

Shipped In

Cold Packs

Safety & Storage

Storage Temperature

After reconstitution, the USP25 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Safety Statement

This USP25 antibody is available for research use only.

Regulatory & Compliance

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