FDFT1 / Squalene synthase catalyzes the first step in the cholesterol biosynthetic pathway, the conversion of trans-farnesyldiphosphate to squalene. The loss of promoter activity and response to sterols for FDFT1 is localized to a 69-bp section positioned 131 bp 5-prime to the transcription start site. Sequence analysis of this region shows that it contains a sterol regulatory element-1 (SRE1) previously identified in other sterol regulated genes and 2 putative NF1 binding sites.
FDFT1 / Squalene synthase catalyzes the first step in the cholesterol biosynthetic pathway, the conversion of trans-farnesyldiphosphate to squalene. The loss of promoter activity and response to sterols for FDFT1 is localized to a 69-bp section positioned 131 bp 5-prime to the transcription start site. Sequence analysis of this region shows that it contains a sterol regulatory element-1 (SRE1) previously identified in other sterol regulated genes and 2 putative NF1 binding sites.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Aliquot the Squalene synthase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Safety Statement
This Squalene synthase antibody is available for research use only.
This Squalene synthase antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
WB, IHC-P
HOST SPECIES
Rabbit
ISOTYPE
Rabbit Ig
REACTIVITY SPECIES
Human
CLONALITY
Polyclonal (rabbit origin)
PURIFICATION
Purified
IMMUNOGEN
A portion of amino acids 140-170 from the human protein was used as the immunogen for the Squalene synthase antibody.
STORAGE BUFFER
In 1X PBS, pH 7.4, with 0.09% sodium azide
APPLICATION DETAILS
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
APPLICATION NOTES
The stated application concentrations are suggested starting points. Titration of the Squalene synthase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
LOCALIZATION
Cytoplasmic
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Aliquot the Squalene synthase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Safety Statement
This Squalene synthase antibody is available for research use only.
Regulatory & Compliance
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We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.