Vimentin Antibody
SKU / CAT#: F54035-0.1ML
$37900
usd
$379.00
F54035-0.1ML

Vimentin Antibody

NSJ Bioreagents
SKU / CAT#: F54035-0.1ML
MPN: F54035-0.1ML
$37900
usd
$379.00
Available
SHIPS Jun 15th
Available
SHIPS Jun 15th
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Description
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Vimentin antibody at -20°C.
Safety Statement
This Vimentin antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Specifications
CLASS
Primary Antibodies
*USAGE / SAFETY STATEMENT
This Vimentin antibody is available for research use only.
FORMAT
Purified
SUBCLASS
Monoclonal
UNIPROT ID
P08670
CLONE NUMBER
4F8-A5-H5
APPLICATION.1
WB, ICC/IF, IP
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG2a
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein G affinity
IMMUNOGEN
A human recombinant partial protein was used as the immunogen for this Vimentin antibody.
STORAGE BUFFER
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
APPLICATION DETAILS
Western blot: 1:1000,ICC/Immunofluorescence: 1:800,Immunoprecipitation: suitable
APPLICATION NOTES
The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Citations (0)
Western blot testing of Vimentin-positive cell lines (Molt-4, K562, COS7, Jurkat, HeLa) and Vimentin-negative cell lines (Daudi, Ramos, Raji) using Vimentin antibody at 1:1000. Predicted molecular weight ~58 kDa.
Western blot testing of Vimentin-positive cell lines (Molt-4, K562, COS7, Jurkat, HeLa) and Vimentin-negative cell lines (Daudi, Ramos, Raji) using Vimentin antibody at 1:1000. Predicted molecular weight ~58 kDa.
Immunoprecipitation of Vimentin from HeLa cell lysate, and subsequent western blot testing, using Vimentin antibody.
ICC/IF staining of HeLa cells, fixed with 4% Paraformaldehyde, using Vimentin antibody at 1:800.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Store the Vimentin antibody at -20°C.
Safety Statement
This Vimentin antibody is available for research use only.
Regulatory & Compliance
Badge
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