The rabbit antibody for calbindin D-28k is generated against calbindin D-28k purified from bovine cerebellum. The antiserum has been shown to detect calbindin D-28k in rat, monkey, human, and baboon. The antibody is provided as 100 µL of...
The rabbit antibody for calbindin D-28k is generated against calbindin D-28k purified from bovine cerebellum. The antiserum has been shown to detect calbindin D-28k in rat, monkey, human, and baboon. The antibody is provided as 100 µL of...
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-20°C
Safety Statement
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records. This product is intended only for laboratory research and development purposes.
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Specifications
CLASS
Primary
COUNTRY OF ORIGIN
United States of America
*USAGE / SAFETY STATEMENT
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records. This product is intended only for laboratory research and development purposes.
REGULATORY NOTICE & RESTRICTIONS
For Laboratory Reagent Use Only. Analytical and performance characteristics are not established. THIS PRODUCT IS FOR RESEARCH USE ONLY AND IS NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE.
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Our lab was able to use this for a beautiful differentiation of the Accumbens core and shell. The protocol followed was essentially identical to the other review...
David
Rutgers University
For our manuscript, Differential roles of ventral pallidum subregions during cocaine self-administration behaviors, in press at Journal of Comparative Neurology,...
David
Rutgers University
Our lab was able to use this for a beautiful differentiation of the Accumbens core and shell. The protocol followed was essentially identical to the other review...
David
Rutgers University
For our manuscript, Differential roles of ventral pallidum subregions during cocaine self-administration behaviors, in press at Journal of Comparative Neurology,...
David
Rutgers University
Core/Shell Differentiation
David B.
Rutgers University
Our lab was able to use this for a beautiful differentiation of the Accumbens core and shell. The protocol followed was essentially identical to the other review written for this product. However, we found that a dilution of 1:5000 was best for our purposes. It should be noted that we use a potassium ferrocyanide counterstain that seems to lighten Calbindin staining. Thus, my pilot studies would suggest that a dilution of 1:7000 would be sufficient for those that are not using such a counterstain. The differences between 1:5000 and 1:7000 dilutions (as tested in increments of 500) were quite subtle, but did make some difference when trying to find the medial edge of the shell under the microscope. I would definitely recommend trying a range of dilutions for those attempting the same accumbens staining. Also, the DAB reaction was quite fast for the calbindin. We only needed 2-4 minutes before it seemed the reaction had reaches asymptote. Feel free to email me for a full protocol.
Visualization of dorsolateral ventral pallidum
David R.
Rutgers University
For our manuscript, Differential roles of ventral pallidum subregions during cocaine self-administration behaviors, in press at Journal of Comparative Neurology, we used the Immunostar rabbit anti-calbindin-d28k primary antibody to visualize the dorsolateral ventral pallidum subregion. Rats were perfused with saline followed by 4% PF, stored in 30% sucrose, and coronally sectioned to 40 um. We followed the protocols of Zahm/Heimer and colleagues when they discovered the ventral pallidum subregions and their afferent/efferent projection patterns.
The protocol consisted of the following steps:
1. Wash in 0.1M phosphate buffer (PB; pH 7.4)
2. 15 min in 1% sodium borohydride
3. Wash
4. 1 hour blocking with PB containing 0.1% Triton X-100 and 3% normal goat serum
5. Primary antibody overnight at 4C - ImmunoStar rabbit anti-calbindin-d28k diluted 1 : 6000 in PB containing 0.1% Triton X-100 and 3% normal goal serum
6. Wash
7. 1 hour anti-rabbit biotinylated secondary (Vector) 1 : 200 in PB with 0.1% Triton X-100
8. Wash
9. 1 hour ABC (Vector) 1 : 200 in PB with 0.1% Triton X-100
10. Wash
11. Develop with 0.05% DAB for 6 min
Our study involved recording neurons within the ventral pallidum subregions during specific aspects of intravenous cocaine self-administration (approaching toward, responding on, or retreating away from a cocaine-reinforced operandum). In order to verify the placement of microwires within the VP subregions, prior to perfusion we passed current through each stainless steel microwire to leave an iron deposit at the uninsulated tip. After DAB (brown reaction) and mounting, we visualized the iron deposit by incubating in a 5% potassium ferrocyanide and 10% HCl solution (leaving a blue-green reaction). For our purposes, this antibody strongly labeled fibers in dorsolateral VP with low background. Individual neurons and other fibers were clearly observed within distinct cortical layers that were not involved in our study.
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Shipped In
Ambient
Safety & Storage
Storage Temperature
-20°C
Safety Statement
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records. This product is intended only for laboratory research and development purposes.
Regulatory & Compliance
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