The rabbit antibody for neuronal nitric oxide synthase C-terminal is generated against a C-terminal synthetic peptide sequence corresponding to amino acids 1419-1433 of human nNOS coupled to KLH. The antibody is provided as 100 uL of lyophilized...
The rabbit antibody for neuronal nitric oxide synthase C-terminal is generated against a C-terminal synthetic peptide sequence corresponding to amino acids 1419-1433 of human nNOS coupled to KLH. The antibody is provided as 100 uL of lyophilized...
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-20°C
Safety Statement
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records. This product is intended only for laboratory research and development purposes.
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Specifications
CLASS
Primary
COUNTRY OF ORIGIN
United States of America
*USAGE / SAFETY STATEMENT
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records. This product is intended only for laboratory research and development purposes.
REGULATORY NOTICE & RESTRICTIONS
For Laboratory Reagent Use Only. Analytical and performance characteristics are not established. THIS PRODUCT IS FOR RESEARCH USE ONLY AND IS NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE.
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We have used this antibody to stain for nNOS positive neurons (also stains some processes) in frozen tissue sections and whole mount preparations of mouse intestine....
Meenakshi
Columbia University Medical Center
We have used this antibody to stain for nNOS positive neurons (also stains some processes) in frozen tissue sections and whole mount preparations of mouse intestine....
Meenakshi
Columbia University Medical Center
Fantastic antibody!
Meenakshi R.
Columbia University Medical Center
We have used this antibody to stain for nNOS positive neurons (also stains some processes) in frozen tissue sections and whole mount preparations of mouse intestine. We get consistent staining at 1:1500 although the staining of the neurons is so strong this can probably be dropped, even for quantification purposes!Tissue prep: 4% PFA/PBS for 1.5 hrs, cryoprotected in 30% sucrose until they sink, OCT embedded, and sectioned. For whole mounts, we open up intestine and fix in 4% PFA/PBS for 1.5 hrs. IF protocol: Sections: Rehydrate in PBS, block with HINGS, stain in primary @4C O/N. Wmts: Wash in PBST, stain in primary with HINGS and DMSO @4C 48 hrs. Sections: Wash with PBS, stain with Alexafluor secondaries (Invitrogen), wash with PBS, and DAPI mount. Wmts: Wash in PBST, stain in secondary with HINGS and DMSO @RT 48 hrs. Optional: proceed with MeOH dehydration and optical clearing.
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Shipped In
Ambient
Safety & Storage
Storage Temperature
-20°C
Safety Statement
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records. This product is intended only for laboratory research and development purposes.
Regulatory & Compliance
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