Vesicular Monoamine Transporter 2 Rabbit Antibody

We have used the VMAT2 antibody from your company a couple of times. From the results it seems that this antibody works very well: it labels the structures in the brain and the spinal cord where this transporter should be located and labeling is very clear.

I have used this antibody (#20042) to label the brains of adult Mouse. Works very well. I highly recommend. I used the antibody on mouse Striatal and substantia Nigra tissue for fluorescence microscopy. I tried different concentration and 1:2000 works the best. The staining was also done in transgenic mice that express GFP on TH promoter for a positive control. The entire process was done on free-floating sections by keeping sections on the shaker agitating and slow speed. DAY 1 Mice were cardiac perfused with fresh 4% Paraformaldehyde and 15% picric acid in 0.1M PB and post fix in same fixative overnight at 4ºC. DAY 2 -Washed brain 3 x 10min in phosphate buffered saline (PBS; pH 7.4) -Brains were cut @ 50um on vibratome -Clean sections with 1% sodium borohydride in PBS for 15 min @ RT, followed by 3 x 10min PBS wash. -Quenched endogenous reaction with 10% Methanol and 3% Hydrogen peroxide in PBS for 15 min @ RT, followed by 3 x 10min PBS wash. -Blocked with 3% bovine serum albumin (BSA), 10% Normal Donkey serum (NDS) and 0.5% Triton X in PBS 4-6 hours @ RT. DAY 3 - Incubate in primary (Rb anti-VMAT2, 1:2000; Immunostar #20042) in 1% BSA, 1% NDS and 0.1% Triton-X in PBS for 60-70 hours @ 4ºC (This is over the weekend. For fast result, can also incubate for overnight @ RT). DAY 4 or 6 -Wash 3 x 10 min in PBS - Incubate in secondary (Donkey Anti Rabbit IgG with Alexa 594, 1:400) for 4-6 hours @ RT -Wash 3 x 10min in PBS -Mount section and cover slip