Misfolded SOD1 Mouse Monoclonal Antibody (A5C3). Superoxide dismutase 1 (SOD1) is a soluble cytoplasmic and mitochondrial intermembrane space protein. SOD1 binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. Mutations in SOD1 cause familial amyotrophic lateral sclerosis type 1 (ALS1). These mutations have been linked to accumulation of harmful superoxide radicals, promotion of apoptosis, formation of aggregates of misfolded superoxide dismutase which are toxic and the continued stimulation of nerve cells that causes them to...
Misfolded SOD1 Mouse Monoclonal Antibody (A5C3). Superoxide dismutase 1 (SOD1) is a soluble cytoplasmic and mitochondrial intermembrane space protein. SOD1 binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. Mutations in SOD1 cause familial amyotrophic lateral sclerosis type 1 (ALS1). These mutations have been linked to accumulation of harmful superoxide radicals, promotion of apoptosis, formation of aggregates of misfolded superoxide dismutase which are toxic and the continued stimulation of nerve cells that causes them to...
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
This product is lyophilized and shipped at room temperature, even if the vial label reads “store at +4℃ or -20℃”. If the product is shipped at ambient temperature, it is known to be stable for the duration of shipping and normal handling. However, storage in the refrigerator or freezer is often recommended for long-term stability of the product. Upon receipt, we recommend storage in the refrigerator or freezer (as indicated in the data sheet of the product).
COUNTRY OF ORIGIN
Canada
APPLICATION
WB, IP, IF
CLONE NUMBER
A5C3
*USAGE / SAFETY STATEMENT
Research Use Only
FORMAT
Lyophilized protein G purified in PBS pH7.4
HOST SPECIES
Mouse
ISOTYPE
IgG1
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal
DILUTION
Each tube contains 25 µg of purified antibodies. If reconstituted with deionized water in 200 µL: IF 1:50 to 1:500; WB 1:250; IP 5 µL / 40 µL (Protein G beads / 300 µg of protein lysate). Optimal dilution must be determined by the user.
IMMUNOGEN
Recombinant human SOD1 G93A APO
PRESERVATIVE
None
SPECIFICITY
The antibody recognizes misfolded forms of mutant human SOD1 protein. By IP and IF, A5C3 is specific for misfolded forms of SOD1 in G93A mutant model compared to normal SOD1. A5C3 clone is also able to recognize different conformers of misfolded SOD1 under specific conditions detailed in various publications.
Target
Misfolded SOD1
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
Safety Statement
Research Use Only
Regulatory & Compliance
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.