Clathrin Light Chain (CLCa / CLCb) Rabbit Polyclonal Antibody. Clathrin-coated vesicles (CCVs) are the major carriers for uptake of nutrients and signalling receptors and for the reformation of synaptic vesicles. Pathogens co-opt the endocytic pathway to gain access into cells. Clathrin is the major component of CCVs and is composed of clathrin-heavy chain and clathrin-light chain (CLC). CLC is a reliable marker of clathrin-coated structures and is also used as a marker of the presynaptic nerve terminal in neurons. Both CLCa and CLCb have alternatively spliced isoforms that are differentially...
Clathrin Light Chain (CLCa / CLCb) Rabbit Polyclonal Antibody. Clathrin-coated vesicles (CCVs) are the major carriers for uptake of nutrients and signalling receptors and for the reformation of synaptic vesicles. Pathogens co-opt the endocytic pathway to gain access into cells. Clathrin is the major component of CCVs and is composed of clathrin-heavy chain and clathrin-light chain (CLC). CLC is a reliable marker of clathrin-coated structures and is also used as a marker of the presynaptic nerve terminal in neurons. Both CLCa and CLCb have alternatively spliced isoforms that are differentially...
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
This product is lyophilized and shipped at room temperature, even if the vial label reads “store at +4℃ or -20℃”. If the product is shipped at ambient temperature, it is known to be stable for the duration of shipping and normal handling. However, storage in the refrigerator or freezer is often recommended for long-term stability of the product. Upon receipt, we recommend storage in the refrigerator or freezer (as indicated in the data sheet of the product).
COUNTRY OF ORIGIN
Canada
APPLICATION
WB, IF
*USAGE / SAFETY STATEMENT
Research Use Only
FORMAT
Lyophilized immunogen affinity purified in PBS pH7.4
HOST SPECIES
Rabbit
REACTIVITY SPECIES
Human, Mouse, Rat
CLONALITY
Polyclonal
DILUTION
If reconstituted with deionized water in 200 µL: IF 1:100 to 1:500; WB 1:1000-1:2000. Optimal dilution has to be determined by the user.
IMMUNOGEN
peptide in N term
PRESERVATIVE
None
SPECIFICITY
The antibody recognizes the N-terminal portion of Clathrin Light Chain
Target
Clathrin Light Chain (CLCa / CLCb)
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Citations (0)
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
Safety Statement
Research Use Only
Regulatory & Compliance
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