GenCRISPR™ Ultra NLS-Cas9-basic GMP is utilized for CRISPR gene editing applications. The Cas9 nuclease forms a stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component. With the help of nuclear localization signal (NLS) expressed with the Cas9 nuclease, the RNP complex enters the nucleus and cleaves the target gene. When compared with a plasmid-based delivery system, the RNP delivery system has been observed to increase the on-target gene editing efficiency and decrease off-target effects.
GenCRISPR™ Ultra NLS-Cas9-basic GMP is utilized for CRISPR gene editing applications. The Cas9 nuclease forms a stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component. With the help of nuclear localization signal (NLS) expressed with the Cas9 nuclease, the RNP complex enters the nucleus and cleaves the target gene. When compared with a plasmid-based delivery system, the RNP delivery system has been observed to increase the on-target gene editing efficiency and decrease off-target effects.
Shipping & Handling
Shipped In
DRY ICE
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Regulatory & Compliance
Specifications
CLASS
Proteins, Enzymes
COUNTRY OF ORIGIN
China
*USAGE / SAFETY STATEMENT
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
PURITY
≥ 95% as analyzed by SDS-PAGE<br>≥ 95% as analyzed by SEC-HPLC<br>
SOURCE
Recombinant Cas9 nuclease (wt) with an
N-terminal NLS expressed in <i>E.coli</i><b> </b><br>
CONCENTRATION
10
mg/mL<br>
SIZE
1mg
APPLICATION.1
sgRNA-dependent
double-stranded DNA cleavage.<br>
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Shipping & Handling
Shipped In
DRY ICE
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Regulatory & Compliance
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