Description
CD274, also known as B7-H1 and PD-L1, is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes.
CD274, also known as B7-H1 and PD-L1, is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes.
Specifications
COUNTRY OF ORIGIN
United Kingdom
*USAGE / SAFETY STATEMENT
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
PURITY
>95% by SDS-PAGE and HPLC
FORMULATION
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
APPLICATION.1
Flow Cytometry, Blocking, IHC (Frozen), Immunofluorescence, Functional Assays
PURIFICATION
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
STORAGE BUFFER
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
APPLICATION NOTES
Each investigator should determine their own optimal working dilution for specific applications.
SPECIFICITY
Mouse anti-mouse PD-L1 antibody (10F.9G2) is a recombinant antibody where the original rat IgG2b backbone of 10F.9G2 has been changed to Mouse IgG1. This antibody recognizes an epitope on Mouse CD274
Antigen Distribution
The CD274 antigien is present on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes
Aggregation
Aggregation level ≤ 5%
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
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