Specifications
COUNTRY OF ORIGIN
United Kingdom
*USAGE / SAFETY STATEMENT
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
PURITY
>95% by SDS-PAGE and HPLC
FORMULATION
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
APPLICATION.1
Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, ELISA
IMMUNOGEN
This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1.
STORAGE BUFFER
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
APPLICATION NOTES
Blocking: Clone MAR1-5A3 has a short half-life, basically because every cell expresses the IFNAR1 receptor and the receptor recycles very rapidly. And, if you want to block function in vivo, you need to be sure that all of the receptors are blocked continually in all compartments. Therefore, you need a large in vivo loading dose (2.5 mg/mouse) to saturate all the binding sites in vivo and then maintain a high enough level to keep them saturated. For in vivo blocking studies we recommend give a loading dose of 2.5 mg/mouse and follow with a weekly dose of 0.5 mg/mouse. The half-live following a 2.5 mg loading dose is about 5 days. [However, if you only inject a low dose of 250 micrograms, then the half life is 1.5 days – because you haven’t saturated the mouse].
Each investigator should determine their own optimal working dilution for specific applications.
SPECIFICITY
Anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) recognizes the extracellular domain of the IFNAR1 subunit of the mouse IFN-alpha / beta receptor.
Antigen Distribution
IFNAR1 and IFNAR2 are coexpressed on nearly all cells.
Aggregation
Aggregation level ≤ 5%
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
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