Specifications
COUNTRY OF ORIGIN
United Kingdom
*USAGE / SAFETY STATEMENT
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
PURITY
>95% by SDS-PAGE and HPLC
CONCENTRATION
1.0 - 5.0 mg/ml
APPLICATION.1
Western Blot, Functional Assays, Flow Cytometry, Immunocytochemistry, IHC (Frozen), Immunoprecipitation
PURIFICATION
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
FORMULATION
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
IMMUNOGEN
Mouse thymus or spleen
SPECIFICITY
Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) recognizes an epitope on mouse CD8b.2 and antibody clone 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1
STORAGE BUFFER
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
APPLICATION NOTES
Each investigator should determine their own optimal working dilution for specific applications.
Antigen Distribution
Majority of thymocytes, cytotoxic T cell subsets
Aggregation
Aggregation level ≤ 5%
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
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