Bulk Anti-Human CD8 (UCHT-4) Antibody

United Kingdom

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

>95% by SDS-PAGE and HPLC

5mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

UCHT-4

Flow Cytometry, Western Blot, Immunoprecipitation, Functional Assays, IHC, Immunofluorescence, ELISA

Mouse

Mouse IgG2a

Human

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Each investigator should determine their own optimal working dilution for specific applications.

Anti-CD8 In Vivo Antibody - Low Endotoxin (UCHT-4) recognizes Human CD8. Anti-Human CD8 recognizes a T-cell glycoprotein cell surface complex (Mr 32 kDa) which defines the cytotoxic/suppressor population of mature T-lymphocytes. The CD8 complex interacts with class I MHC molecules resulting in increased adhesion between CD8+ T-lymphocytes and their target cells. Binding of the CD8 antigen to Class I MHC molecules enhances T-cell activation. The cytoplasmic portion of the CD8 antigen is associated with a protein tyrosine kinase (p56lck).

CD8

The CD8 antigen is expressed on approximately 19-48% of normal human peripheral blood lymphocytes and > 90% of normal thymocytes. It is also present on a subset of NK cells. Subjects infected with HIV have been shown to exhibit increased levels of CD8+ lymphocytes with a continual decrease in CD4+ lymphocytes.

Aggregation level ≤ 5%

<1.0 EU/mg as determined by the LAL method