MMP9 active protein (prokaryotic), Pig

Cloud-Clone Corp. Wuhan

SKU / CAT#: APA553Po01-1mg

^$4,428⁰⁰

usd

^$4,428.00/mg

APA553Po01-1mg

MMP9 active protein (prokaryotic), Pig

Name: MMP9 active protein (prokaryotic), Pig
Price: 4428.00 USD
Availability: InStock

SKU / CAT#: APA553Po01-1mg

MPN:

^$4,428⁰⁰

usd

^$4,428.00/mg

Available

SHIPS Jul 31st

Available

SHIPS Jul 31st

SKU

Unit size / Options

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Description

MMP9 active protein (prokaryotic), Pig

Shipping & Handling

Shipped In

Cold packs

Safety & Storage

Storage Temperature

Store at 2-8℃ for one month,-80℃ for 12 months

Safety Statement

Research Use Only

Regulatory & Compliance

Technical Documents

MANUAL (1)

Product Manual for MMP9 active protein (prokaryotic), Pig

Specifications

CLASS

Active proteins

SHIPPING NOTES

FedEx Service

COUNTRY OF ORIGIN

China

RAW MATERIAL ORIGINS

China

APPLICATION

Cell culture; Activity Assays.

USAGE

Reconstitute in PBS or others.

GENE ID

https://www.ncbi.nlm.nih.gov/gene/654325

UNIPROT ID

Q2VI03

*USAGE / SAFETY STATEMENT

Research Use Only

PURITY

Greater than 90% by SDSPAGE

pH RANGE

7.4

CONCENTRATION

0.2mg/mL

MWCO (kDa)

30kDa

VALIDATED APPLICATION

Cell culture; Activity Assays.

SOURCE

E.coli

HOST SPECIES

E.coli

REACTIVITY SPECIES

Pig

CROSS REACTIVITY

Pig

Questions & Answers (0)

Citations (0)

Figure. Hydrolysis of gelatin by MMP9.<br/>Mechanism: MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant pig MMP9 (1000ng, 500ng, 100ng, 10ng, 1ng, 0.1ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate- polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP9 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP9 protein as negative control, and blood sample as positive control. <br/>Result: Gelatin hydrolysis by recombinant pig MMP9 (10-70kd) was shown in figure 1.

Shipping & Handling

Shipped In

Cold packs

Safety & Storage

Storage Temperature

Store at 2-8℃ for one month,-80℃ for 12 months

Safety Statement

Research Use Only

Regulatory & Compliance

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