Rat Estradiol ELISA Kit

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

China

Competitive ELISA

Estradiol

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Rat

Colorimetric

< 0.422 ng/ml

0.703 - 45 ng/ml

Plasma, tissue homogenates and other biological fluids.

This Rat Estradiol ELISA Kit is an in vitro competitive enzyme-linked immunosorbent assay for the quantitative measurement of Estradiol in plasma, tissue homogenates and other biological fluids.<br></br>A microplate has been precoated with Estradiol. Samples, standards and Anti-Estradiol Antibody (Biotin) are added to the wells, where Estradiol in the sample competes with the fixed Estradiol to bind to the antibody. The wells are washed to remove unbound material and HRP-conjugated streptavidin is added. After incubation, the wells are washed again and TMB substrate is added. The TMB substrate is catalyzed by HRP to produce blue colouration. The reaction is terminated by addition of Stop Solution which stops the colour development and produces a colour change from blue to yellow. The intensity of the signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.

Competitive

Microplate

94%