Human EGF ELISA Kit

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

China

EGF

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Human

Colorimetric

< 1 pg/ml

4.7-300 pg/ml

Serum, body fluids, tissue lysates, and cell culture supernatants.

This EGF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-EGF Antibody. Standards or samples are then added to the microtire plate wells and EGF, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of EGF present in the sample, Anti-EGF Antibody (Biotin) is added to each well to "sandwich" the EGF immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain EGF will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Sandwich (quantitative)

Microplate