Human Interleukin-13 Receptor Alpha 2 ELISA Kit

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

Canada

Sandwich ELISA

Interleukin-13 Receptor Alpha 2

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Human

This assay has shown no cross-reactivity with other cytokines and growth factors tested.

Colorimetric

< 10 pg/ml

This sandwich ELISA recognises both natural and recombinant human IL-13 Ra2.

10-500 pg/ml

Serum, plasma and cell culture supernatant.

This assay applies the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific to IL-13 Rα2 has been pre-coated onto a microplate. Standards and samples are then added to the appropriate plate wells with a biotin - conjugated antibody preparation specific for IL-13 Rα2 and incubated. IL-13 Rα2 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. After washing away any unbound substances, avidin-Horseradish Peroxidase (HRP) will be added to each well and incubated. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and only those wells that contain IL-13 Rα2, biotin conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The colour development is stopped and the intensity of the colour is measured. <br></br>In order to measure the concentration of IL-13 Rα2 in the samples, this package of reagent includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-13 Rα2 concentration. The concentration of IL-13 Rα2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sandwich (quantitative)

Microplate