Human Granzyme B ELISA Kit

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

Sweden

ELISA

Granzyme B

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Human

Colorimetric

5 pg/ml

10-3160 pg/ml

Serum, plasma, and cell culture supernatant.

This Human Granzyme B ELISA Kit applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for Granzyme B. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Granzyme B if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound Granzyme B and other components of the sample. A biotinylated Anti-Granzyme B detection antibody is then added followed by Streptavidin-HRP. Addition of TMB substrate will result in a colored substrate product. The reaction is stopped with sulfuric acid and the optical density can be quantified using an ELISA plate reader. The concentration of analyte is determined by comparison to a serial dilution of the ELISA standard in parallel.

Sandwich (quantitative)

Microplate