Anti-NuMA Antibody [A73-B/D12]

Primary

Monoclonal

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

FC, IF, IHC-P

A73-B/D12

NuMA

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Liquid

200 µg/ml

Mouse

IgM

Human

Monoclonal

Unconjugated

PEG precipitation.

FC: 1-2 µg / million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Colon carcinoma 174T cells.

Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

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