Anti-AKT1 Antibody [AKT1/2491]

Primary

Monoclonal

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

WB, IHC-P

AKT1/2491

AKT1

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Liquid

200 µg/ml

Mouse

IgG2b

Human

Monoclonal

Unconjugated

Protein A/G chromatography.

WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Recombinant fragment, around amino acids 85-189, of human AKT1 protein. The exact sequence is proprietary.

Recognizes a protein of 62kDa, which is identified as AKT1The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

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