Mouse IL-17E ELISA Kit

Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).

United States of America

Sandwich ELISA

IL-17E

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Mouse

This assay has shown no significant cross-reactivity.

Colorimetric

1.22 pg/ml

This kit can assay both natural and recombinant mouse IL-17E.

62.50 - 4000 pg/ml

Interleukin 17E (IL-17E), also known as IL-25, is a cytokine that belongs to the IL-17 cytokine family and is secreted by type 2 helper T cells (Th2) and mast cells. It shares the sequence similarity with IL-17. This cytokine can induce NF-κB activation, and stimulate the production of IL-8. Both this cytokine and IL-17B are ligands for the cytokine receptor IL-17RB. Studies in mice suggested that this cytokine may be a proinflammatory cytokine favoring Th2-type immune response. IL-17E induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, which stimulate the expansion of eosinophils. This cytokine is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. IL-17E can kill some types of breast cancer cells.

Cell culture supernatant, serum and plasma.

Mouse IL-17E ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-17E in the samples. A monoclonal antibody specific for mouse IL-17E has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-17E, and IL-17E present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-17E bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Sandwich (quantitative)

Microplate

83% (Average Recovery from Serum Samples).