Specifications
SHIPPING NOTES
Storing antibodies at various temperatures (up to 40°C) for one week does not affect their activity. As a precautionary measure, we ship all products in insulated packaging with cold packs to provide extra temperature stability in transit. The cold packs may be thawed when you receive the shipment, please be assured that this is normal and your product(s) are safe to use. Once you have received your product(s), please follow the storage instructions on the datasheet(s).
APPLICATION
WB, ICC/IF, IHC
*USAGE / SAFETY STATEMENT
This product is for research use only. It is not intended for diagnostic or therapeutic use.
MWCO (kDa)
345 kDa, 395 kDa
REACTIVITY SPECIES
Human, Rat, Mouse
CONJUGATION/TAG
Unconjugated
DILUTION
WB: 1:5,000-1:10,000, ICC/IF: 1:2,000-1:5,000, IHC: 1:1,000
IMMUNOGEN
Recombinant construct containing amino acids 1,111-1,490 of human Ki67 isotype 1, the central region of both Ki67 isotypes including the 2nd, 3rd, and 4th Ki67 repeat domains, expressed in and purified from E. coli.
STORAGE BUFFER
Supplied as an aliquot of serum with 5mM Sodium Azide.
GENERAL NOTES
Please note, Ki67 proteins are very unstable and only expressed in large amounts in situations where many cells are dividing. As a result of the very short half life of Ki67 there are usually numerous fragments visible on western blots running below the major 395 kDa and 345 kDa bands.