Specifications
GENE ID
https://www.ncbi.nlm.nih.gov/gene/1956
*USAGE / SAFETY STATEMENT
This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.
DISCLAIMER
This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.
REACTIVITY SPECIES
Human, Mouse, Rat
PURIFICATION
Antigen-affinity purified from serum by sequential phospho-peptide and non-phospho-peptide affinity columns
EPITOPE
A short sequence around phosphorylated T654 (exact sequence is proprietary)
IMMUNOGEN
A synthetic peptide around phosphorylated T678 of human EGFR (NP_005219.2) corresponding to T654 of the mature protein (starts with L25).
POSITIVE CONTROL
rat liver
FORMULATION (WITHOUT BSA)
1 mg IgG/ml in PBS, 0.02% azide, 50% glycerol
ALTERNATIVE NAME
Epithelial Growth Factor Receptor; EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA
CELL. LOCALIZATION
cell surface, plasma membrane, translocation to nucleus upon EGF stimulation
WB
AE00300 to EGFR-pT654 was successfully used to stain an approx. 150kDa band in mouse and rat liver lysates. Recommended concentration: 1-3ug/ml
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