EpCAM Monoclonal Antibody AE00150

Monoclonal

ICC, IHC, PA, WB

https://www.ncbi.nlm.nih.gov/gene/4072

This product is for in vitro research use only. Any other applications, such as diagnostics or therapeutics, or in vivo experiments, and the validation of this product therein, are solely at the responsibility of the buyer/user.

This product is only warranted for the specifications as described in this product sheet and only when the product is handled and stored according to instructions. User should validate this antibody in the application and tissue/cell type as required, after confirmation of integrity upon receipt is obtained by reproducing the performance as described below. Should such confirmation not be attempted, any warranty is void. In case of non-conformance, user needs to contact us immediately for replacement or refund.

Mab

Mouse

IgG1

Human

By Protein G from bioreactor concentrate

Extracellular domain (within aa 77-202 region)

200ug IgG/ml in PBS, 0.05% BSA, 0.05% azide

Recombinant fragment around aa 77-202 of human EpCAM protein (exact sequence is proprietary)

A431, carcinoma, HCT116, HEK293, lung, MCF7

EGP40/1372

1mg IgG/ml in PBS

EpCAM

Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial Cell Adhesion Molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD326; DIAR5; EGP; EGP-2; EGP314; EGP40; Ep-CAM; ESA; hEGP314; HNPCC8; KS1/4; KS 1/4 antigen; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1

EPCAM

82

cell surface, plasma membrane

AB_2744511

AE00150 to EpCAM was successfully used to stain cellular membranes in human colorectal and hepatocellular carcinoma sections. Recommended concentration: 0.3-1ug/ml

AE00150 to EpCAM was successfully used to stain an approx. 40kDa band in human lung lysates and approx. 35kDa in lysates of cell lines HEK293, A431 and HCT116. Recommended concentration: 0.5-1.5ug/ml

AE00150 to EpCAM was successfully used to stain cellular membranes in cell line MCF7 and in human colorectal carcinoma. Recommended concentration: 1-3ug/ml

SDS-PAGE Analysis of Purified EpCAM Mouse Monoclonal Antibody AE00150. Confirmation of Purity and Integrity of Antibody.

Specificity and selectivity of AE00150 to EpCAM were tested against >19,000 full-length human proteins on a human protein array. A protein BLAST search against H. sapiens revealed the following closely related other protein: TROP2. This protein was part of the array used and showed no cross-reactivity signals.