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Direct-zol RNA Miniprep

Zymo Research

SKU / CAT#: R2050

^$186⁰⁰

usd

^$3.72/preps

R2050

Direct-zol RNA Miniprep

Name: Direct-zol RNA Miniprep
Price: 186.00 USD
Availability: InStock
Rating: 4.75 (6 reviews)

Zymo Research Corporation

36reviews

20citationscitations

31questionsquestions

SKU / CAT#: R2050

MPN: R2050

^$186⁰⁰

usd

^$3.72/preps

Available

SHIPS May 29th

Available

SHIPS May 29th

SKU

Unit size / Options

Availability

Price

No products found

Description

The Direct-zol™ RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including...

Shipping & Handling

Shipped In

Ambient

Safety & Storage

Storage Temperature

See product data sheet, protocol and/or package labeling for storage conditions.

Safety Statement

This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.

Regulatory & Compliance

Technical Documents

Datasheet (1)

Direct-zol RNA Miniprep - Data Sheet

MSDS (1)

Direct-zol RNA Miniprep - SDS (MSDS)

PROTOCOL (1)

Direct-zol RNA Miniprep - Protocol

Specifications

CLASS

RNA

SUBCLASS

RNA Isolation

SHIPPING NOTES

All orders received before 3:00 pm Pacific Standard Time Monday through Friday will be shipped the same day via FedEx® Ground delivery unless otherwise stated or requested.

COUNTRY OF ORIGIN

United States of America

*USAGE / SAFETY STATEMENT

This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.

SOURCE

Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).

KIT COMPONENTS

● DNA Digestion Buffer (E1010-1-4). _x000D_● DNA Digestion Buffer (E1010-1-16). _x000D_● TRI Reagent (R2050-1-200). _x000D_● TRI Reagent (R2050-1-50). _x000D_● Zymo-Spin IICR Columns (C1078-50). _x000D_● RNA Wash Buffer (R1003-3-12). _x000D_● RNA Wash Buffer (R1003-3-48). _x000D_● DNase I Set (E1010). _x000D_● Direct-zol RNA PreWash (Concentrate) (R2050-2-40). _x000D_● Direct-zol RNA PreWash (Concentrate) (R2050-2-160). _x000D_● Collection Tubes (C1001-50). _x000D_● Direct-zol RNA Miniprep (R2050). _x000D_● Direct-zol RNA Miniprep (R2052). _x000D_● DNase/RNase-Free Water (W1001-6). _x000D_● DNase/RNase-Free Water (W1001-30). _x000D_

EQUIPMENT

Microcentrifuge, vortex

SIZE RANGE

Total RNA ≥ 17 nt

YIELD

50 µg RNA (binding capacity), ≥25 µl (elution volume)

Questions & Answers (0)

Reviews & Ratings (6)

6 REVIEWS

4.8 out of 5 stars

50%

The kit procedures are easy and the price is competitive. However, I did not have luck with processing my tissue directly in Trizol solution as the kit intended....

Tom

University of Missouri

We normally use Tri-reagent when extracting RNA from tissues (we work with poultry viruses and extract RNA from different tissues such as trachea, cecal tonsils,...

Cassandra

Auburn University

Direct-zol™ RNA MiniPrep is so effective. I need purified samples of total RNA from hepatic tissue as well as tumor-derived cells and in both cases, I obtain highly...

Isabel

Instituto de Neurobiología, UNAM

Good deal. It works great. We use it for our brain samples and it has given us good results so far. We have contacted the sales team and found them to be helpful...

BHUVANA

Oklahoma state University

Very efficient for gram positive bacteria when combine with bead beating. Easy to use. Fast extraction leading to high quality RNA.

Charlotte

CSU

Direct-zol RNA MiniPrep kits are easy to use and we get consistent results from our RNA extractions. It is also more cost effective than other kits weve used.

Andy

Montana State University

The kit procedures are easy and the price is competitive. However, I did not have luck with processing my tissue directly in Trizol solution as the kit intended....

Tom

University of Missouri

We normally use Tri-reagent when extracting RNA from tissues (we work with poultry viruses and extract RNA from different tissues such as trachea, cecal tonsils,...

Cassandra

Auburn University

Direct-zol™ RNA MiniPrep is so effective. I need purified samples of total RNA from hepatic tissue as well as tumor-derived cells and in both cases, I obtain highly...

Isabel

Instituto de Neurobiología, UNAM

Good deal. It works great. We use it for our brain samples and it has given us good results so far. We have contacted the sales team and found them to be helpful...

BHUVANA

Oklahoma state University

Very efficient for gram positive bacteria when combine with bead beating. Easy to use. Fast extraction leading to high quality RNA.

Charlotte

CSU

Direct-zol RNA MiniPrep kits are easy to use and we get consistent results from our RNA extractions. It is also more cost effective than other kits weve used.

Andy

Montana State University

Feb. 16, 2022

Good price for kit that can quickly yield DNA free RNA.

Tom C.

University of Missouri

The kit procedures are easy and the price is competitive. However, I did not have luck with processing my tissue directly in Trizol solution as the kit intended. Yield was unusable. I did have success and good yield with the same kit when processing the samples from the aqueous phase of Trizol. For the cost and ease of kit which includes DNAse, I do recommend the kit for RNA isolation but for me I will not be using the direct in Trizol technique.

Jan. 31, 2022

Very helpful kit for RNA extraction, clean RNA.

Cassandra K.

Auburn University

We normally use Tri-reagent when extracting RNA from tissues (we work with poultry viruses and extract RNA from different tissues such as trachea, cecal tonsils, spleen, harderian gland, etc). I tried this kit to help streamline the process and get cleaner RNA. I like that I do not have to worry about losing the pellet during the extraction process because this uses columns and the RNA quality is very good.

Jan. 26, 2022

Satisfied with this product

Isabel M.

Instituto de Neurobiología, UNAM

Direct-zol™ RNA MiniPrep is so effective. I need purified samples of total RNA from hepatic tissue as well as tumor-derived cells and in both cases, I obtain highly pure RNA that serves as a template for RT-qPCR. I have high reproducibility between qPCR assays. This kit is easy and ready to use. The extraction and purification are fast, following instructions without the possibility of contamination. The customer service after-sales is really efficient and kind. I think this kit offers excellent results to obtain high-quality samples for a just price.

Citations (0)