Direct-zol RNA Miniprep
Zymo Research
SKU / CAT#: R2050
$18600
usd
$3.72/preps
R2050

Direct-zol RNA Miniprep

Zymo Research Corporation
$18600
usd
$3.72/preps
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Available
SHIPS May 7th
Available
SHIPS May 7th
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Description
The Direct-zol™ RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including...
The Direct-zol™ RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including...
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
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Specifications
CLASS
RNA
SUBCLASS
RNA Isolation
SHIPPING NOTES
All orders received before 3:00 pm Pacific Standard Time Monday through Friday will be shipped the same day via FedEx® Ground delivery unless otherwise stated or requested.
COUNTRY OF ORIGIN
United States of America
*USAGE / SAFETY STATEMENT
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
SOURCE
Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
KIT COMPONENTS
● DNA Digestion Buffer (E1010-1-4). _x000D_● DNA Digestion Buffer (E1010-1-16). _x000D_● TRI Reagent (R2050-1-200). _x000D_● TRI Reagent (R2050-1-50). _x000D_● Zymo-Spin IICR Columns (C1078-50). _x000D_● RNA Wash Buffer (R1003-3-12). _x000D_● RNA Wash Buffer (R1003-3-48). _x000D_● DNase I Set (E1010). _x000D_● Direct-zol RNA PreWash (Concentrate) (R2050-2-40). _x000D_● Direct-zol RNA PreWash (Concentrate) (R2050-2-160). _x000D_● Collection Tubes (C1001-50). _x000D_● Direct-zol RNA Miniprep (R2050). _x000D_● Direct-zol RNA Miniprep (R2052). _x000D_● DNase/RNase-Free Water (W1001-6). _x000D_● DNase/RNase-Free Water (W1001-30). _x000D_
EQUIPMENT
Microcentrifuge, vortex
SIZE RANGE
Total RNA ≥ 17 nt
YIELD
50 µg RNA (binding capacity), ≥25 µl (elution volume)
Questions & Answers (0)
Reviews & Ratings (6)
4.8 out of 5 stars
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The kit procedures are easy and the price is competitive. However, I did not have luck with processing my tissue directly in Trizol solution as the kit intended....
Tom
University of Missouri
We normally use Tri-reagent when extracting RNA from tissues (we work with poultry viruses and extract RNA from different tissues such as trachea, cecal tonsils,...
Cassandra
Auburn University
Direct-zol™ RNA MiniPrep is so effective. I need purified samples of total RNA from hepatic tissue as well as tumor-derived cells and in both cases, I obtain highly...
Isabel
Instituto de Neurobiología, UNAM
Good deal. It works great. We use it for our brain samples and it has given us good results so far. We have contacted the sales team and found them to be helpful...
BHUVANA
Oklahoma state University
Very efficient for gram positive bacteria when combine with bead beating. Easy to use. Fast extraction leading to high quality RNA.
Charlotte
CSU
Direct-zol RNA MiniPrep kits are easy to use and we get consistent results from our RNA extractions. It is also more cost effective than other kits weve used.
Andy
Montana State University
The kit procedures are easy and the price is competitive. However, I did not have luck with processing my tissue directly in Trizol solution as the kit intended....
Tom
University of Missouri
We normally use Tri-reagent when extracting RNA from tissues (we work with poultry viruses and extract RNA from different tissues such as trachea, cecal tonsils,...
Cassandra
Auburn University
Direct-zol™ RNA MiniPrep is so effective. I need purified samples of total RNA from hepatic tissue as well as tumor-derived cells and in both cases, I obtain highly...
Isabel
Instituto de Neurobiología, UNAM
Good deal. It works great. We use it for our brain samples and it has given us good results so far. We have contacted the sales team and found them to be helpful...
BHUVANA
Oklahoma state University
Very efficient for gram positive bacteria when combine with bead beating. Easy to use. Fast extraction leading to high quality RNA.
Charlotte
CSU
Direct-zol RNA MiniPrep kits are easy to use and we get consistent results from our RNA extractions. It is also more cost effective than other kits weve used.
Andy
Montana State University
Good price for kit that can quickly yield DNA free RNA.
Tom C.
University of Missouri
The kit procedures are easy and the price is competitive. However, I did not have luck with processing my tissue directly in Trizol solution as the kit intended. Yield was unusable. I did have success and good yield with the same kit when processing the samples from the aqueous phase of Trizol. For the cost and ease of kit which includes DNAse, I do recommend the kit for RNA isolation but for me I will not be using the direct in Trizol technique.
Very helpful kit for RNA extraction, clean RNA.
Cassandra K.
Auburn University
We normally use Tri-reagent when extracting RNA from tissues (we work with poultry viruses and extract RNA from different tissues such as trachea, cecal tonsils, spleen, harderian gland, etc). I tried this kit to help streamline the process and get cleaner RNA. I like that I do not have to worry about losing the pellet during the extraction process because this uses columns and the RNA quality is very good.
Satisfied with this product
Isabel M.
Instituto de Neurobiología, UNAM
Direct-zol™ RNA MiniPrep is so effective. I need purified samples of total RNA from hepatic tissue as well as tumor-derived cells and in both cases, I obtain highly pure RNA that serves as a template for RT-qPCR. I have high reproducibility between qPCR assays. This kit is easy and ready to use. The extraction and purification are fast, following instructions without the possibility of contamination. The customer service after-sales is really efficient and kind. I think this kit offers excellent results to obtain high-quality samples for a just price.
Citations (0)
Direct-zol RNA Miniprep
Direct-zol RNA Miniprep
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
Badge
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