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EZ DNA Methylation-Lightning Kit
Zymo Research
SKU / CAT#: D5031
$54700
usd
$2.73/Rxns.
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D5031

EZ DNA Methylation-Lightning Kit

Zymo Research Corporation
268reviews
268
20citationscitations
20
15questionsquestions
15
SKU / CAT#: D5031
MPN: D5031
$54700
usd
$2.73/Rxns.
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Available
SHIPS Jun 9th
Available
SHIPS Jun 9th
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Description
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
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Technical Documents
Datasheet (1)
EZ DNA Methylation-Lightning Kit - Data Sheet
MSDS (2)
EZ DNA Methylation-Lightning Kit - SDS (MSDS)
EZ DNA Methylation-Lightning Kit - SDS (MSDS)
PROTOCOL (1)
EZ DNA Methylation-Lightning Kit - Protocol
ADDITIONAL (4)
EZ DNA Methylation-Lightning Kit
EZ DNA Methylation-Lightning Kit
EZ DNA Methylation-Lightning Kit
EZ DNA Methylation-Lightning Kit
Specifications
CLASS
Epigenetics
SUBCLASS
DNA Methylation
SHIPPING NOTES
All orders received before 3:00 pm Pacific Standard Time Monday through Friday will be shipped the same day via FedEx® Ground delivery unless otherwise stated or requested.
COUNTRY OF ORIGIN
United States of America
*USAGE / SAFETY STATEMENT
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
SOURCE
Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
INPUT
100 pg - 2 µg of DNA.
KIT COMPONENTS
● Zymo-Spin IC Columns (C1004-50). _x000D_● Collection Tubes (C1001-50). _x000D_● M-Wash Buffer (D5002-4). _x000D_● M-Wash Buffer (D5001-4). _x000D_● M-Binding Buffer (D5005-3). _x000D_● M-Elution Buffer (D5002-6). _x000D_● M-Elution Buffer (D5001-6). _x000D_● M-Binding Buffer (D5006-3). _x000D_● L-Desulphonation Buffer (D5030-5). _x000D_● L-Desulphonation Buffer (D5031-5). _x000D_● Lightning Conversion Reagent (D5030-1). _x000D_
ELUTION VOLUME
≥ 10 µl
EQUIPMENT
Thermocycler with heated lid and microcentrifuge.
Questions & Answers (0)
Reviews & Ratings (6)
4.8 out of 5 stars
5
67%
4
33%
3
0%
2
0%
1
0%
Its an easy of use product, good balance between value and money
Rene
univerisity
I had used this back in India and the results are always consistent and reproducible. But never had a chance to test with primary and cell lines. But here I have...
Kathirvel
University of Georgia
Easy to use, with great results.
Sofia
UNAM
Very useful product, will purchase again!
Zirui
Virginia Tech
Compared to other RNA isolation kits, the Direct-zol RNA miniprep plus maximizes the amount of RNA isolated from a small sample size. The ability to go directly...
Robert
National Institute on Aging, NIH
Great results! Easy to use, very reproducible
JIANHUA
Johns Hopkins University
Its an easy of use product, good balance between value and money
Rene
univerisity
I had used this back in India and the results are always consistent and reproducible. But never had a chance to test with primary and cell lines. But here I have...
Kathirvel
University of Georgia
Easy to use, with great results.
Sofia
UNAM
Very useful product, will purchase again!
Zirui
Virginia Tech
Compared to other RNA isolation kits, the Direct-zol RNA miniprep plus maximizes the amount of RNA isolated from a small sample size. The ability to go directly...
Robert
National Institute on Aging, NIH
Great results! Easy to use, very reproducible
JIANHUA
Johns Hopkins University
Mar. 31, 2022
It is good
Rene N.
univerisity
Its an easy of use product, good balance between value and money
Mar. 31, 2022
Impressive results and easy to use and worry free
Kathirvel M.
University of Georgia
I had used this back in India and the results are always consistent and reproducible. But never had a chance to test with primary and cell lines. But here I have tried on both and surprisingly the results are great and reproducible. I love it. Its amazingly simple and easy to use.
Mar. 30, 2022
Good results in short time
Sofia P.
UNAM
Easy to use, with great results.
Citations (0)
EZ DNA Methylation-Lightning Kit
EZ DNA Methylation-Lightning Kit
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
Badge
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