ZymoPURE II Plasmid Midiprep Kit
Zymo Research
SKU / CAT#: D4200
$27800
usd$11.12/Preps.
ZymoPURE II Plasmid Midiprep Kit
Zymo Research CorporationSKU / CAT#: D4200
MPN: D4200
$27800
usd$11.12/Preps.
Available
SHIPS Aug 4th
Available
SHIPS Aug 4th
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Description
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 18 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with either a vacuum or a centrifuge instead of...
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 18 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with either a vacuum or a centrifuge instead of...
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
Technical Documents
Specifications
CLASS
DNA
SUBCLASS
Bacterial Plasmid
SHIPPING NOTES
All orders received before 3:00 pm Pacific Standard Time Monday through Friday will be shipped the same day via FedEx® Ground delivery unless otherwise stated or requested.
COUNTRY OF ORIGIN
United States of America
APPLICATION
Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications.
*USAGE / SAFETY STATEMENT
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
PURITY
Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0
FORMAT
Spin-Column
INPUT
≤ 50 ml
KIT COMPONENTS
● ZymoPURE P1 (Red) (D4200-1-3). _x000D_● ZymoPURE P1 (Red) (D4200-1-13). _x000D_● ZymoPURE P1 (Red) (D4200-1-100). _x000D_● ZymoPURE P1 (Red) (D4200-1-150). _x000D_● ZymoPURE P1 (Red) (D4200-1-210). _x000D_● ZymoPURE P1 (Red) (D4200-1-410). _x000D_● ZymoPURE P2 (Green) (D4200-2-3). _x000D_● ZymoPURE P2 (Green) (D4200-2-13). _x000D_● ZymoPURE P2 (Green) (D4200-2-100). _x000D_● ZymoPURE P2 (Green) (D4200-2-150). _x000D_● ZymoPURE P2 (Green) (D4200-2-210). _x000D_● ZymoPURE P2 (Green) (D4200-2-410). _x000D_● ZymoPURE P3 (Yellow) (D4200-3-3). _x000D_● ZymoPURE P3 (Yellow) (D4200-3-13). _x000D_● ZymoPURE P3 (Yellow) (D4200-3-100). _x000D_● ZymoPURE P3 (Yellow) (D4200-3-150). _x000D_● ZymoPURE P3 (Yellow) (D4200-3-210). _x000D_● ZymoPURE P3 (Yellow) (D4200-3-410). _x000D_● ZymoPURE Binding Buffer (D4200-4-3). _x000D_● ZymoPURE Binding Buffer (D4200-4-14). _x000D_● ZymoPURE Binding Buffer (D4200-4-110). _x000D_● ZymoPURE Binding Buffer (D4200-4-150). _x000D_● ZymoPURE Binding Buffer (D4200-4-210). _x000D_● ZymoPURE Binding Buffer (D4200-4-410). _x000D_● ZymoPURE Wash 1 (D4200-5-20). _x000D_● ZymoPURE Wash 1 (D4200-5-55). _x000D_● ZymoPURE Wash 1 (D4200-5-320). _x000D_● ZymoPURE Wash 1 (D4200-5-410). _x000D_● ZymoPURE Wash 2 (Concentrate) (D4200-6-10). _x000D_● ZymoPURE Wash 2 (Concentrate) (D4200-6-12). _x000D_● ZymoPURE Wash 2 (Concentrate) (D4200-6-23). _x000D_● ZymoPURE Wash 2 (Concentrate) (D4200-6-28). _x000D_● ZymoPURE Elution Buffer (D4200-7-1). _x000D_● ZymoPURE Elution Buffer (D4200-7-6). _x000D_● ZymoPURE Elution Buffer (D4200-7-12). _x000D_● ZymoPURE Elution Buffer (D4200-7-30). _x000D_● Collection Tubes (C1001-50). _x000D_● Collection Tubes (C1001-500). _x000D_● Collection Tubes (C1001-1000). _x000D_● ZymoPURE Syringe Filter-X (C1092-5). _x000D_● ZymoPURE Syringe Plungers (C1037-5). _x000D_● Zymo-Spin V-PS Column Assembly w/15 ml Reservoir-X and 50 ml Reservoir (C1083-5). _x000D_● EndoZero II Spin Columns (C1060-25). _x000D_
ELUTION VOLUME
≥ 150 µl
EQUIPMENT
Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge
SIZE RANGE
Up to 200 kb
YIELD
Up to 1.2 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 50 ml of overnight culture grown in LB for high copy number plasmids are 200 – 400 µg.
Questions & Answers (0)
Reviews & Ratings (6)
4.7 out of 5 stars
5
100%
4
0%
3
0%
2
0%
1
0%
This is the product we have been using for years.
Nicole D.
JHMI
Easy to use and high yields. Has the option to remove the endotoxin.
Fabulous!
Anna E.
University of California Berkeley
The Zymo midi/maxi kits are great! It would be even better if the syringe filters/columns could fit into 50 mL conicals.
Great product.
Hashnu D.
CSIR-Indian Institute of Chemical Technology
The sales team is very good and the product is wonderful.
Citations (0)
Shipping & Handling
Shipped In
Ambient
Safety & Storage
Storage Temperature
See product data sheet, protocol and/or package labeling for storage conditions.
Safety Statement
This product is for research use only and should only be used by trained professionals. It is not for use in diagnostic procedures.
Regulatory & Compliance
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