POLE3 Antibody / CHRAC-17
SKU / CAT#: V9234-100UG
$49900
usd
$4.99/UG
V9234-100UG

POLE3 Antibody / CHRAC-17

NSJ Bioreagents
SKU / CAT#: V9234-100UG
MPN: V9234-100UG
$49900
usd
$4.99/UG
Available
SHIPS Jun 19th
Available
SHIPS Jun 19th
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Description
DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular...
DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular...
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Safety & Storage
Storage Temperature
Aliquot the POLE3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Safety Statement
This POLE3 antibody is available for research use only.
Regulatory & Compliance
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Technical Documents
Specifications
CLASS
Primary Antibodies
SUBCLASS
Monoclonal
UNIPROT ID
Q9NRF9
CLONE NUMBER
PCRP-POLE3-2F10
*USAGE / SAFETY STATEMENT
This POLE3 antibody is available for research use only.
FORMAT
Purified
APPLICATION.1
FACS, IF, IHC-P
HOST SPECIES
Mouse
ISOTYPE
Mouse IgG1
REACTIVITY SPECIES
Human
CLONALITY
Monoclonal (mouse origin)
PURIFICATION
Protein A/G affinity
IMMUNOGEN
Recombinant full-length human DNA polymerase epsilon subunit 3 protein was used as the immunogen for the POLE3 antibody.
STORAGE BUFFER
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
APPLICATION DETAILS
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
APPLICATION NOTES
Optimal dilution of the POLE3 antibody should be determined by the researcher.
LOCALIZATION
Nucleus, Cytoplasm
Questions & Answers (0)
Citations (0)
IHC staining of FFPE human lymphoma tissue with POLE3 antibody (clone PCRP-POLE3-2F10) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymphoma tissue with POLE3 antibody (clone PCRP-POLE3-2F10) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS staining of PFA-fixed human K562 cells with POLE3 antibody (blue, clone PCRP-POLE3-2F10) and isotype control (red).
+1
SDS-PAGE analysis of purified, BSA-free POLE3 antibody (clone PCRP-POLE3-2F10) as confirmation of integrity and purity.
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
Aliquot the POLE3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Safety Statement
This POLE3 antibody is available for research use only.
Regulatory & Compliance
Badge
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